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cell culture 852 human embryonic kidney hek 293ft cells  (ATCC)


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    ATCC cell culture 852 human embryonic kidney hek 293ft cells
    Cell Culture 852 Human Embryonic Kidney Hek 293ft Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 37945 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+human+embryonic+kidney+hek+293t+cells/293T/pm42015511-356-2-11
    Average 99 stars, based on 37945 article reviews
    cell culture 852 human embryonic kidney hek 293ft cells - by Bioz Stars, 2026-09
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    Cell Culture:

    Article Title: Prime Editing of Vascular Endothelial Growth Factor Receptor 2 Attenuates Angiogenesis In Vitro .
    Article Snippet: Vascular endothelial growth factor receptor (VEGFR)-2 is a key switch for angiogenesis, which is observed in various human diseases.. In this study, a novel system for advanced prime editing (PE), termed PE6h, is developed, consisting of dual lentiviral vectors: (1) a clustered regularly interspaced palindromic repeatassociated protein 9 (H840A) nickase fused with reverse transcriptase and an enhanced PE guide RNA and (2) a dominant negative (DN) MutL homolog 1 gene with nicking guide RNA.. PE6h was used to edit VEGFR2 (c.18315T>A, 50.8%) to generate a premature stop codon (TAG from AAG), resulting in the production of DN-VEGFR2 (787 aa) in human retinal microvascular endothelial cells (HRECs).

    Article Title: Effect of pH, temperature and freezing-thawing on quantity changes and cellular uptake of exosomes
    Article Snippet: .. Cell culture Human embryonic kidney (HEK) 293T cells were from American Type Culture Collection (ATCC) (Manassas, VA, USA). .. Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; HyClone, SH30243.01) containing 10% fetal bovine serum (FBS; LONSA, S711-001S) or exo-free FBS and 1% Penicillin-streptomycin (PS; HyClone, SV30010) at 37°C in a humidified incubator (Thermo, Forma Series II) containing 5% CO2.

    Article Title: Global DNA methylation analysis reveals miR-214-3p contributes to cisplatin resistance in pediatric intracranial nongerminomatous malignant germ cell tumors
    Article Snippet: .. Cell Culture Human embryonic kidney (HEK)293T cells were obtained from the American Type Culture Collection. ..

    Article Title: 5′-end NAD + cap in human cells promotes RNA decay through DXO-mediated deNADding
    Article Snippet: .. Cell Culture Human embryonic kidney (HEK) 293T cells were obtained from ATCC. .. Cells were cultured in DMEM medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum, penicillin–streptomycin under 5% CO2 at 37°C.

    Article Title: GSDMB promotes non-canonical pyroptosis by enhancing caspase-4 activity
    Article Snippet: Images were taken with a high-resolution digital camera (Olympus DP 50). .. Cell culture Human embryonic kidney (HEK) 293T cells and 293FT cells were obtained from the ATCC. ..

    Article Title: Functional Characterization of Melanocortin-3 Receptor In Rainbow Trout (Oncorhynchus Mykiss)
    Article Snippet: .. 2.7 Cell culture Human embryonic kidney (HEK) 293T cells, obtained from American Type Culture Collection (ATCC, Manassas, VA, USA), were cultured in Dulbecco’s modi ed Eagle’s medium (DMEM, Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS, Biological Industries, Kibbutz Beit Haemek, Israel) at 370C in 5% CO2-humidi ed atmosphere. ..

    Article Title: Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics
    Article Snippet: .. Cell culture Human embryonic kidney (HEK) 293T cells (CRL-11268, American Type Culture Collection) and mouse embryonic fibroblasts (MEFs) were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich) that was supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin, under standard cell culture conditions (37 °C, humidified, 5% CO2/95% air). ..

    Article Title: Magnesium protects against sepsis by blocking gasdermin D N-terminal-induced pyroptosis
    Article Snippet: .. Cell culture Human embryonic kidney (HEK) 293T cells and 293FT cells were obtained from ATCC and were cultured in Dulbecco’s modified Eagle’s medium (DMEM). ..

    Modification:

    Article Title: Prime Editing of Vascular Endothelial Growth Factor Receptor 2 Attenuates Angiogenesis In Vitro .
    Article Snippet: Vascular endothelial growth factor receptor (VEGFR)-2 is a key switch for angiogenesis, which is observed in various human diseases.. In this study, a novel system for advanced prime editing (PE), termed PE6h, is developed, consisting of dual lentiviral vectors: (1) a clustered regularly interspaced palindromic repeatassociated protein 9 (H840A) nickase fused with reverse transcriptase and an enhanced PE guide RNA and (2) a dominant negative (DN) MutL homolog 1 gene with nicking guide RNA.. PE6h was used to edit VEGFR2 (c.18315T>A, 50.8%) to generate a premature stop codon (TAG from AAG), resulting in the production of DN-VEGFR2 (787 aa) in human retinal microvascular endothelial cells (HRECs).

    Article Title: Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics
    Article Snippet: .. Cell culture Human embryonic kidney (HEK) 293T cells (CRL-11268, American Type Culture Collection) and mouse embryonic fibroblasts (MEFs) were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich) that was supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin, under standard cell culture conditions (37 °C, humidified, 5% CO2/95% air). ..

    Article Title: Magnesium protects against sepsis by blocking gasdermin D N-terminal-induced pyroptosis
    Article Snippet: .. Cell culture Human embryonic kidney (HEK) 293T cells and 293FT cells were obtained from ATCC and were cultured in Dulbecco’s modified Eagle’s medium (DMEM). ..



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    ATCC cell culture human embryonic kidney hek 293 t cells
    ALDH1L1 interacts with and degrades PEDV N and E proteins via the selective autophagy pathway. ( A and B ) <t>HEK</t> <t>293T</t> cells were transfected with plasmids coding HA-N or HA-E and Flag-ALDH1L1. Co-IP assay was performed using anti-Flag-bound beads. ( C and D ) After inducing Flag-ALDH1L1 and GST-N or GST-E expression in the BL21(DE3) bacterial strain, the associations of ALDH1L1 with N or E proteins were analyzed via the GST pull-down assay. ( E and F ) After transfection with HA-N or HA-E and Flag-ALDH1L1, HeLa cells were labeled with antibodies and nuclear DAPI for confocal immunofluorescence microscopy. Scale bars = 100 µm. ( G and H ) After transfecting HEK 293T cells with varying concentrations of Flag-ALDH1L1, HA-N, or HA-E, western blotting was performed to assess N and E protein expression. ( I and J ) HEK 293T cells were transfected with plasmids coding the Flag-ALDH1L1 plasmid and HA-N or HA-E, followed by treatment with BafA1 (50 µM), CQ (50 µM), MG132 (5 µM), or 3-MA (1 mM) for 10 h. The resulting cellular lysates were subjected to western blotting.
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    cell culture human embryonic kidney hek 293t cells  (ATCC)
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    ATCC cell culture human embryonic kidney hek 293t cells
    ALDH1L1 interacts with and degrades PEDV N and E proteins via the selective autophagy pathway. ( A and B ) <t>HEK</t> <t>293T</t> cells were transfected with plasmids coding HA-N or HA-E and Flag-ALDH1L1. Co-IP assay was performed using anti-Flag-bound beads. ( C and D ) After inducing Flag-ALDH1L1 and GST-N or GST-E expression in the BL21(DE3) bacterial strain, the associations of ALDH1L1 with N or E proteins were analyzed via the GST pull-down assay. ( E and F ) After transfection with HA-N or HA-E and Flag-ALDH1L1, HeLa cells were labeled with antibodies and nuclear DAPI for confocal immunofluorescence microscopy. Scale bars = 100 µm. ( G and H ) After transfecting HEK 293T cells with varying concentrations of Flag-ALDH1L1, HA-N, or HA-E, western blotting was performed to assess N and E protein expression. ( I and J ) HEK 293T cells were transfected with plasmids coding the Flag-ALDH1L1 plasmid and HA-N or HA-E, followed by treatment with BafA1 (50 µM), CQ (50 µM), MG132 (5 µM), or 3-MA (1 mM) for 10 h. The resulting cellular lysates were subjected to western blotting.
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    ALDH1L1 interacts with and degrades PEDV N and E proteins via the selective autophagy pathway. ( A and B ) HEK 293T cells were transfected with plasmids coding HA-N or HA-E and Flag-ALDH1L1. Co-IP assay was performed using anti-Flag-bound beads. ( C and D ) After inducing Flag-ALDH1L1 and GST-N or GST-E expression in the BL21(DE3) bacterial strain, the associations of ALDH1L1 with N or E proteins were analyzed via the GST pull-down assay. ( E and F ) After transfection with HA-N or HA-E and Flag-ALDH1L1, HeLa cells were labeled with antibodies and nuclear DAPI for confocal immunofluorescence microscopy. Scale bars = 100 µm. ( G and H ) After transfecting HEK 293T cells with varying concentrations of Flag-ALDH1L1, HA-N, or HA-E, western blotting was performed to assess N and E protein expression. ( I and J ) HEK 293T cells were transfected with plasmids coding the Flag-ALDH1L1 plasmid and HA-N or HA-E, followed by treatment with BafA1 (50 µM), CQ (50 µM), MG132 (5 µM), or 3-MA (1 mM) for 10 h. The resulting cellular lysates were subjected to western blotting.

    Journal: Journal of Virology

    Article Title: ALDH1L1 suppresses the replication of porcine epidemic diarrhea virus by degrading viral nucleocapsid and envelope proteins

    doi: 10.1128/jvi.01933-25

    Figure Lengend Snippet: ALDH1L1 interacts with and degrades PEDV N and E proteins via the selective autophagy pathway. ( A and B ) HEK 293T cells were transfected with plasmids coding HA-N or HA-E and Flag-ALDH1L1. Co-IP assay was performed using anti-Flag-bound beads. ( C and D ) After inducing Flag-ALDH1L1 and GST-N or GST-E expression in the BL21(DE3) bacterial strain, the associations of ALDH1L1 with N or E proteins were analyzed via the GST pull-down assay. ( E and F ) After transfection with HA-N or HA-E and Flag-ALDH1L1, HeLa cells were labeled with antibodies and nuclear DAPI for confocal immunofluorescence microscopy. Scale bars = 100 µm. ( G and H ) After transfecting HEK 293T cells with varying concentrations of Flag-ALDH1L1, HA-N, or HA-E, western blotting was performed to assess N and E protein expression. ( I and J ) HEK 293T cells were transfected with plasmids coding the Flag-ALDH1L1 plasmid and HA-N or HA-E, followed by treatment with BafA1 (50 µM), CQ (50 µM), MG132 (5 µM), or 3-MA (1 mM) for 10 h. The resulting cellular lysates were subjected to western blotting.

    Article Snippet: Dulbecco’s modified Eagle’s medium (Sigma-Aldrich, D6429) supplemented with 10% fetal bovine serum (Gibco, 10099141) was used to culture human embryonic kidney HEK 293T cells (ATCC, CRL-11268) and African green monkey kidney Vero cells (ATCC, CCL-81).

    Techniques: Transfection, Co-Immunoprecipitation Assay, Expressing, Pull Down Assay, Labeling, Immunofluorescence, Microscopy, Western Blot, Plasmid Preparation

    ALDH1L1 inhibits PEDV replication via the E3 ubiquitin ligase STUB1. ( A ) After HEK 293T cells were cotransfected with plasmids encoding HA-STUB1 and Flag-ALDH1L1, the Co-IP assay was performed using anti-Flag-bound beads. ( B ) After HEK 293T cells were transfected with plasmids coding Flag-ALDH1L1, Co-IP assays were performed using an anti-Flag antibody. ( C ) After inducing GST-STUB1 and ALDH1L1 expression in the BL21(DE3) strain, the interaction between ALDH1L1 and STUB1 was examined via the GST pull-down assay. ( D ) After cotransfecting HeLa cells with Flag-ALDH1L1 and STUB1-HA for 24 h via incubation with an anti-Flag Mab, ALDH1L1 and STUB1 colocalization was confirmed via confocal immunofluorescence microscopy. Scale bars = 100 µm. ( E and F ) After cotransfection with Myc-ALDH1L1, HA-Ub, HA-STUB1, and Flag-N, or Flag-E, HEK 293T cells were treated with CQ and collected. The ubiquitinated N and E proteins were subjected to western blotting after immunoprecipitation with an anti-Flag antibody. ( G and H ) After cotransfecting HEK 293T cells with siRNAs (negative control or STUBA siRNA) and HA-N or HA-E and Flag-ALDH1L1-coding plasmids, western blotting was performed with an anti-HA antibody.

    Journal: Journal of Virology

    Article Title: ALDH1L1 suppresses the replication of porcine epidemic diarrhea virus by degrading viral nucleocapsid and envelope proteins

    doi: 10.1128/jvi.01933-25

    Figure Lengend Snippet: ALDH1L1 inhibits PEDV replication via the E3 ubiquitin ligase STUB1. ( A ) After HEK 293T cells were cotransfected with plasmids encoding HA-STUB1 and Flag-ALDH1L1, the Co-IP assay was performed using anti-Flag-bound beads. ( B ) After HEK 293T cells were transfected with plasmids coding Flag-ALDH1L1, Co-IP assays were performed using an anti-Flag antibody. ( C ) After inducing GST-STUB1 and ALDH1L1 expression in the BL21(DE3) strain, the interaction between ALDH1L1 and STUB1 was examined via the GST pull-down assay. ( D ) After cotransfecting HeLa cells with Flag-ALDH1L1 and STUB1-HA for 24 h via incubation with an anti-Flag Mab, ALDH1L1 and STUB1 colocalization was confirmed via confocal immunofluorescence microscopy. Scale bars = 100 µm. ( E and F ) After cotransfection with Myc-ALDH1L1, HA-Ub, HA-STUB1, and Flag-N, or Flag-E, HEK 293T cells were treated with CQ and collected. The ubiquitinated N and E proteins were subjected to western blotting after immunoprecipitation with an anti-Flag antibody. ( G and H ) After cotransfecting HEK 293T cells with siRNAs (negative control or STUBA siRNA) and HA-N or HA-E and Flag-ALDH1L1-coding plasmids, western blotting was performed with an anti-HA antibody.

    Article Snippet: Dulbecco’s modified Eagle’s medium (Sigma-Aldrich, D6429) supplemented with 10% fetal bovine serum (Gibco, 10099141) was used to culture human embryonic kidney HEK 293T cells (ATCC, CRL-11268) and African green monkey kidney Vero cells (ATCC, CCL-81).

    Techniques: Ubiquitin Proteomics, Co-Immunoprecipitation Assay, Transfection, Expressing, Pull Down Assay, Incubation, Immunofluorescence, Microscopy, Cotransfection, Western Blot, Immunoprecipitation, Negative Control

    ALDH1L1 suppresses PEDV replication via the cargo receptor TOLLIP. ( A ) After cotransfecting HEK 293T cells with plasmids coding HA-TOLLIP and Flag-ALDH1L1, a Co-IP assay was performed with anti-Flag-bound beads. ( B ) After transfecting HEK 293T cells with plasmids encoding Flag-ALDH1L1, Co-IP assays were performed using an anti-Flag antibody. ( C ) After inducing GST-TOLLIP and ALDH1L1 expression in the BL21(DE3) strain, the interaction between ALDH1L1 and TOLLIP was examined via the GST pull-down assay. ( D ) HeLa cells were transfected with plasmids coding Flag-ALDH1L1 and HA-TOLLIP. Then, the cells were incubated with specific antibodies and visualized under a confocal immunofluorescence microscope. Scale: 100 µm. ( E and F ) After cotransfecting HEK 293T cells with siRNAs (negative control or TOLLIP siRNA) and HA-N or HA-E and Flag-ALDH1L1-coding plasmids, western blotting was performed using an anti-HA antibody.

    Journal: Journal of Virology

    Article Title: ALDH1L1 suppresses the replication of porcine epidemic diarrhea virus by degrading viral nucleocapsid and envelope proteins

    doi: 10.1128/jvi.01933-25

    Figure Lengend Snippet: ALDH1L1 suppresses PEDV replication via the cargo receptor TOLLIP. ( A ) After cotransfecting HEK 293T cells with plasmids coding HA-TOLLIP and Flag-ALDH1L1, a Co-IP assay was performed with anti-Flag-bound beads. ( B ) After transfecting HEK 293T cells with plasmids encoding Flag-ALDH1L1, Co-IP assays were performed using an anti-Flag antibody. ( C ) After inducing GST-TOLLIP and ALDH1L1 expression in the BL21(DE3) strain, the interaction between ALDH1L1 and TOLLIP was examined via the GST pull-down assay. ( D ) HeLa cells were transfected with plasmids coding Flag-ALDH1L1 and HA-TOLLIP. Then, the cells were incubated with specific antibodies and visualized under a confocal immunofluorescence microscope. Scale: 100 µm. ( E and F ) After cotransfecting HEK 293T cells with siRNAs (negative control or TOLLIP siRNA) and HA-N or HA-E and Flag-ALDH1L1-coding plasmids, western blotting was performed using an anti-HA antibody.

    Article Snippet: Dulbecco’s modified Eagle’s medium (Sigma-Aldrich, D6429) supplemented with 10% fetal bovine serum (Gibco, 10099141) was used to culture human embryonic kidney HEK 293T cells (ATCC, CRL-11268) and African green monkey kidney Vero cells (ATCC, CCL-81).

    Techniques: Co-Immunoprecipitation Assay, Expressing, Pull Down Assay, Transfection, Incubation, Immunofluorescence, Microscopy, Negative Control, Western Blot